Journal:

Article Title: Mandelic Acid Condensation Polymer: Novel Candidate Microbicide for Prevention of Human Immunodeficiency Virus and Herpes Simplex Virus Entry

doi: 10.1128/JVI.76.22.11236-11244.2002

Figure Lengend Snippet: Effects of SAMMA on HIV infection of macrophages and PBMC. Primary macrophages were infected with the R5 laboratory isolate BaL (A) or the R5 clinical isolate BR/92/003 (C) in the presence of the indicated concentrations of SAMMA. Viral replication was monitored by determining supernatant HIV-1 p24 (Gag) protein by ELISA on day 7. (C and D) Primary CD4+ T cells were infected with the X4 laboratory isolate IIIB (B) or the X4 clinical isolate UG021 (D) in the presence or absence of SAMMA, and p24 was quantified on day 5. Results represent the average p24 concentrations from triplicate wells (error bars indicate standard deviation) and are representative of at least two independent experiments.

Article Snippet: Laboratory-adapted HIV-1 strains included the R5 laboratory isolate HIV-1 BAL and the X4 laboratory isolate HIV-1 IIIB (Advanced Biotechnologies Inc, Columbia, Md.).

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal:

Article Title: Mandelic Acid Condensation Polymer: Novel Candidate Microbicide for Prevention of Human Immunodeficiency Virus and Herpes Simplex Virus Entry

doi: 10.1128/JVI.76.22.11236-11244.2002

Figure Lengend Snippet: Viral infection following preincubation of cells or virus for 1 h with SAMMA or control media

Article Snippet: Laboratory-adapted HIV-1 strains included the R5 laboratory isolate HIV-1 BAL and the X4 laboratory isolate HIV-1 IIIB (Advanced Biotechnologies Inc, Columbia, Md.).

Techniques: Infection, Activity Assay

Journal:

Article Title: Mandelic Acid Condensation Polymer: Novel Candidate Microbicide for Prevention of Human Immunodeficiency Virus and Herpes Simplex Virus Entry

doi: 10.1128/JVI.76.22.11236-11244.2002

Figure Lengend Snippet: Effects of SAMMA on HIV-1 gp120-cell membrane interactions. U87MG control, CD4+, CD4+CXCR4+, or CD4+CCR5+ cells were preincubated with medium or 500 μg of SAMMA per ml for 15 min. Then 50 ng of MN gp120 (MG, CD4+, and CD4+CXC4+ cells) or YU2 gp120 (CD4+CCR5+ cells) was added, and the mixture was incubated at 37°C with constant rotation for 1 h and washed extensively. The cell lysates were analyzed on an 8% Tris-glycine gel and then subjected to Western transfer and probing for gp120 using a 1:1,000 dilution of the 1169 polyclonal antiserum. Colorimetric detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG and Western Blue stabilized substrate. Controls include cells alone (lane 1) and recombinant gp120 alone (lane 4). Data are representative of three independent experiments.

Article Snippet: Laboratory-adapted HIV-1 strains included the R5 laboratory isolate HIV-1 BAL and the X4 laboratory isolate HIV-1 IIIB (Advanced Biotechnologies Inc, Columbia, Md.).

Techniques: Incubation, Western Blot, Recombinant